Serological and genetic analysis of a rare CisAB01/O01 blood group
Received:January 11, 2021  Revised:February 18, 2021
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DOI:10.46701/BG.2021012021101
KeyWord:CisAB  serological blood typing  genetic typing  sequencing
                       
AuthorInstitution
Xiaoyong Liu Transfusion Department of Ganzhou City Hospital, Ganzhou, Jiangxi 341000, China
Zhaodi Yi Transfusion Department of Ganzhou City Hospital, Ganzhou, Jiangxi 341000, China
Ming Gao Jiangsu LIBO Medicine Biotechnology Co., Ltd. Jiangyin, Jiangsu 214400, China
Haojun Zhang Jiangsu ZOJIWAT Biomedical Co., Ltd. Jiangyin, Jiangsu 214400, China
Buqiang Wang Transfusion Department of Ganzhou City Hospital, Ganzhou, Jiangxi 341000, China
Hongjun Gao Jiangsu LIBO Medicine Biotechnology Co., Ltd. Jiangyin, Jiangsu 214400, China
Yi Wu Jiangsu ZOJIWAT Biomedical Co., Ltd. Jiangyin, Jiangsu 214400, China
Yuping Chen Jiangsu LIBO Medicine Biotechnology Co., Ltd. Jiangyin, Jiangsu 214400, China
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Abstract:
      The paper aims to analyze a rare blood sample in Ganzhou City Hospital with CisAB subtype and explore a feasible pattern for blood typing of rare blood type patients, so as to ensure clinical transfusion safety. The routine serological methods were used for ABO forward and reverse blood typing and the fluorescence real-time PCR technique was used for sample genotyping. A human ABO blood group 6-7 exon sequencing kit was used for sequence analysis. The nucleic acid sequence of the sample was compared with reference sequences. The forward typing results demonstrated that the sample was ABw, RhD positive. The sample exhibited 4+ agglutination with anti-H and anti-AB antibodies. Reverse typing by microcolumn gel method showed an AB result, but the serum sample demonstrated weak agglutination with B cell under room temperature, 4 °C and 37 °C in saline when tested with tube method respectively. The serological results matched with the A2B3 serotype. The fluorescent real-time PCR genotyping results displayed A/O01. The sequence analysis demonstrated deletion of guanine in 261-position 467C>T (heterozygote) and 803G>T (heterozygote) mutation respectively. The mutation caused the A glycosyltransferase peptide chain to change from proline to leucine (P156L) at 156 and from glutamate to alanine (G268A) at 268. The result demonstrated that the sample's genotype was CisAB01/O01. The mutation of glycosyltransferase coding gene leads to an abnormal serological reaction pattern. Only by combining the results of genetic analysis can we get the true sample blood type and better ensure the safety of clinical blood transfusion.
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