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| Molecular analysis to FUT-1, 2, 3 and ABO genotyping may instead of serological typing to diagnose para-Bombay in Chinese |
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| DOI：10.46701/APJBG.20170217032 |
| KeyWord:para-Bombay, FUT-1, FUT-2, FUT-3, ABO blood group |
| Author | Institution |
| HaoChun Chang, |
Department of Clinical Medicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. |
| XiaoFei Li |
Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China. |
| Yu-Shiang Lin |
Department of Clinical Medicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. |
| Zhiyuan Xu |
Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China. |
| Daowang Fan |
Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China. |
| Yan Qiu |
Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China. |
| Tianhong Miao |
Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China. |
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| Abstract: |
| The aim of this study was to evaluate the consistency between serotyping and molecular analysis in Chinese with para-Bombay. The molecular analysis of gene fragments in FUT-1, FUT-2, FUT-3 and ABO genotyping and serotyping were used including a saliva test to examine the A, B, H substance and an absorption elution test to examine the A, B, H; and further routine tests including ABO, H and Lewis phenotype. From eleven samples with anti-H negative, 10 samples were confirmed with para-Bombay by sequencing to FUT-1, from which six samples were 547-548delAG, three samples were 880TT deletion, one sample was 35C>T and one sample was 649G>T heterozygous (h7, China) as carrier. The sequencing to FUT-2 confirmed 357C>T in 11 samples, meaning H, A and B substance was secreted in saliva except for one sample which occurred 385A>T (I129F) heterozygous, which is a weak secretor. The FUT-3 sequence result demonstrated four samples with heterozygous mutations to 59T>G (L20R) combined with 508G>A (G170S) and seven samples without mutations in FUT-3 gene fragment same as reference. The consistency between sequencing with FUT-1/FUT-2 and serotyping by anti-H reported an identical result, except for one sample, which interestingly showed the H/h7 carrier with serotyping negative to anti-H. The result of sequencing with FUT-2/FUT-3 and Lewis phenotyping also reported a complete consistency. The saliva test to A, B, H substance and absorption elution test examining the A, B, H antigens on the surface of red blood cells completely matched the ABO exon 6, 7 sequence results. The sequencing of FUT-1, FUT-2, FUT-3 and ABO exon 6, 7 may become a useful tool to confirm the para-Bombay blood type. |
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